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KMID : 0370220070510020083
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Yakhak Hoeji
2007 Volume.51 No. 2 p.83 ~ p.87
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Inhibitory Effects of Ethaverine on Serotonin Content in Murine Mastocytoma P815 Cells
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Kim Eung-Il
Lim Sung-Cil
Lee Myung-Koo
Park Seung-Kook
Shin Jung-Soo
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Abstract
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The effects of ethaverine on serotonin biosynthesis in murine mastocytoma P815 cells were investigated. Ethaverine at 2.5{sim}10 {mu}M decreased serotonin content in a concentration-dependent manner. The IC_{50} value of ethaverine was 6.1 {mu}M. Ethaverine at concentrations up to 20 {mu}M was not cytotoxic towards P815 cells. Under these conditions, tryptophan hydroxylase (EC 1.14.16.4; TPH), a rate-limiting enzyme in serotonin biosynthesis, was inhibited by ethaverine in P815 cells (15.3% inhibition at 7.5 {mu}M), however, aromatic L-amino acid decarboxylase (EC 4.1.1.28) was not. These results indicate that ethaverine leads to a decrease in serotonin content by reducing TPH activity in P815 cells.
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KEYWORD
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ethaverine, serotonin content, tryptophan hydroxylase, P815 cells
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